Real-Time Monitoring of the Formation and Culture of Hybrid Cell-Microbiomaterial Spheroids Using Non-Faradaic Electrical Impedance Spectroscopy.

ABSTRACT: Cellular spheroids are considered a popular option for modeling healthy and diseased tissues in vitro and as injectable therapies. The formation and culture of spheroids can make use of different three-dimensional (3D) culture platforms, but the spheroids' analysis often has to rely on endpoint assays. In this study, we propose a microfluidic bioreactor to culture and nondestructively monitor human mesenchymal stem cell (hMSC) spheroids over time using non-Faradaic electr(ochem)ical impedance spectroscopy (EIS). For this, an array of porous microwells thermoformed from ion track-etched thin films and a pair of sensing electrodes from transparent indium tin oxide are integrated into the flow and culture chamber of the bioreactor. To measure the spheroid's electrical properties, the e

SUBMITTER: Fois MG 

PROVIDER: S-EPMC12522083 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature