YAP contributes to liver injury through mediating capillarization of liver sinusoidal endothelial cells via GATA6/eNOS signaling.

ABSTRACT:

Background

Capillarization of liver sinusoidal endothelial cells (LSECs) is a central event in response to liver injury. In this study, we investigated the role of Yes-associated protein 1 (YAP1, also known YAP) in LSEC capillarization and liver injury.

Methods

YAP expression was assessed in liver samples from mice injured by CCl₄ injection and DDC diet. EC-specific Yap1 conditional knockout mice (Yap1^∆end) were generated by breeding Yap1^fl/fl mice with Cdh5-Cre^ERT2 mice. HA-PEI/siYap1 nanoparticles were applied to specifically inhibit YAP expression in LSECs.

Results

YAP was primarily expressed in LSECs, and its expression was elevated during liver fibrosis. EC-specific Yap1 deficiency significantly increased the fenestrae

SUBMITTER: Wang J 

PROVIDER: S-EPMC12522350 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature