Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA.

ABSTRACT:

Background

Aedes albopictus is a primary vector for the transmission of dengue fever. RNA interference (RNAi)-based biocidal technology represents an important alternative and complement to conventional chemically synthesized insecticides.

Methods

Chlorella vulgaris was used as a carrier organism for RNAi-mediated gene silencing of Ae. albopictus. Short hairpin RNA (shRNA) expression vectors targeting the vgat and vmat genes of Ae. albopictus were constructed and subsequently used to transform C. vulgaris. The shRNA-transformed Chlorella were then administered to Ae. albopictus larvae or incorporated into attractive toxic sugar baits (ATSB) for adult feeding. The effects of silencing the vgat and vmat genes on Ae. albopictus were then investigated.

Results

Both vgat a

SUBMITTER: Deng X 

PROVIDER: S-EPMC12522392 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature