Ferrostatin-1, a ferroptosis inhibitor, mitigates all-trans-retinal-induced retinal pigment epithelium degeneration in mice.

ABSTRACT:

Background

Disruption of the retinoid (visual) cycle causes all-trans-retinal (atRAL) to accumulate in photoreceptors and retinal pigment epithelium (RPE), contributing to Stargardt disease type 1 (STGD1) and dry age-related macular degeneration (dAMD). Yet, the mechanisms underlying atRAL-induced RPE degeneration are not well understood. Here, we explored whether ferroptosis, a form of regulated cell death marked by iron-dependent lipid peroxidation, mediated RPE damage by atRAL.

Methods

Cell and animal models of STGD1 and dAMD were established using ARPE-19 cells loaded with atRAL and Abca4^-/-Rdh8^-/- mice subjected to light exposure. Cell viability and morphology, mitochondrial morphology, ferrous iron (Fe²⁺) levels, reactive oxygen species

SUBMITTER: Shen X 

PROVIDER: S-EPMC12522635 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature