Integrated profiling of semen microbiota and metabolome reveals dysregulation in primary idiopathic male infertility.

ABSTRACT:

Background

Primary idiopathic male infertility remains a significant challenge due to its complex etiology and the lack of effective diagnostic tools. This study characterized seminal microbiota and metabolome alterations in this condition and their association with sperm quality, aiming to identify potential biomarkers.

Methods

We conducted an integrated microbiota-metabolome profiling study, enrolling 26 men with primary idiopathic infertility and 14 fertile controls. Semen samples were evaluated according to WHO guidelines. Microbiota composition was assessed by 5R 16S rRNA sequencing, and metabolites were profiled using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Statistical analyses included diversity assessment, differential abu

SUBMITTER: Yao H 

PROVIDER: S-EPMC12522667 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature