H2BC9 lactylation modulates esophageal squamous cell carcinoma progression via the Wnt/β-catenin signaling pathway.

ABSTRACT:

Background

Lactate, traditionally regarded as a metabolic waste product of glycolysis, has recently emerged as a critical signaling molecule and epigenetic modifier via protein lactylation. However, the functional consequences of histone lactylation in cancer progression remain poorly understood.

Methods

We integrated transcriptomic data from TCGA and GEO (GSE188900) with bulk and single-cell RNA-seq analyses to identify lactylation-associated histone variants involved in esophageal squamous cell carcinoma (ESCC). Functional assays, immunoprecipitation (IP), western blotting (WB), chromatin immunoprecipitation (ChIP)-qPCR, and luciferase reporter assays were employed to investigate the role of H2BC9 and its lactylation in ESCC. A xenograft tumor model was used to validate in

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12522879 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature