LncRNA FTX/OCT4/miR-122-5p orchestrates self-renewal and lineage commitment of human dental pulp stem cells by directly targeting FOXO3.

ABSTRACT:

Background

Human dental pulp stem cells (hDPSCs) possess multipotent properties and play a pivotal role in tissue regeneration. Elucidating the molecular mechanisms governing hDPSC pluripotency is essential for advancing their clinical application. We previously demonstrated that long noncoding RNA five prime to Xist (lncRNA FTX) suppresses the proliferation and multilineage differentiation capacity of hDPSCs by inhibiting octamer-binding transcription factor 4 (OCT4). This study aims to explore the molecular mechanisms by which FTX regulates hDPSC proliferation/differentiation via downstream miRNAs and their biological implications.

Methods

Small RNA sequencing was utilized to identify differentially expressed miRNAs between the FTX-overexpressing and vector hDPSCs. Bioinfo

SUBMITTER: Zeng K 

PROVIDER: S-EPMC12522884 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature