FAM19A4 and miR124-2 methylation status in human papillomavirus-driven and human papillomavirus-negative oropharyngeal squamous cell carcinomas.

ABSTRACT:

Background

Scarce data exist on host gene methylation in oropharyngeal squamous cell carcinomas (OPCs), which are caused by human papillomavirus (HPV) in approximately 40% of cases. We analyzed the methylation status of two host genes involved in cervical carcinogenesis, FAM19A4 and miR124-2, in formalin-fixed paraffin-embedded (FFPE) tissues of primary HPV-driven OPCs (i.e., HPV-positive and p16INK4A-positive), HPV-negative OPCs, head and neck (HN) squamous cell papillomas, and oral rinse-and-gargles (ORGs) from individuals without HN lesions.

Methods

A multiplex real-time methylation-specific PCR on bisulfite-converted DNA was employed (PreCursor-M+, Fujirebio). Hypermethylation for each target was expressed as negative/positive based on the ΔΔCt, as well as by the ΔΔCt ra

SUBMITTER: Dona MG 

PROVIDER: S-EPMC12522942 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature