Mechanical isolation of stromal vascular fraction from adipose tissue: methods and cellular outcomes: a systematic review and meta-analysis.

ABSTRACT:

Background

Stromal vascular fraction (SVF) from adipose tissue is a rich and accessible source of regenerative cells, including adipose-derived stem cells (ADSCs). SVF is most commonly isolated from lipoaspirate via enzymatic digestion, a process that is costly and considered 'more than minimal manipulation' by the United States Food and Drug Administration. In contrast, mechanically based isolation techniques have gained attention as a simpler, faster, and regulatory-compliant alternative, making them increasingly appealing for clinical applications.

Main text

This systematic review and meta-analysis aimed to evaluate the outcomes of mechanical methods for harvesting SVF from human adipose tissue. Key parameters assessed included cell yield, viability, surface marker expres

SUBMITTER: Sforza M 

PROVIDER: S-EPMC12522944 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature