Single-cell RNA-seq combined with bulk RNA-seq analysis identifies necroptosis-related genes as therapeutic targets for periodontitis.

ABSTRACT:

Background

Necroptosis, a regulated form of programmed cell death, exacerbates inflammatory responses by releasing damage-associated molecular patterns and inflammatory factors. However, the specific mechanisms underlying necroptosis in periodontitis remain largely unclear. This study integrated single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing (RNA-seq) data to identify core necroptosis-related genes (NRGs) and validated these findings using external datasets and periodontitis samples collected during our research.

Methods

Overlapping genes were identified through a comparative analysis of 114 NRGs sourced from GeneCards and marker genes specific to various cell types in the single-cell GSE171213 periodontitis dataset. Based on these genes, cells were categoriz

SUBMITTER: Zhu F 

PROVIDER: S-EPMC12523025 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature