IL-6⁺ fibroblasts contribute to shaping the pro-inflammatory microenvironment in severe periodontitis after non-surgical periodontal therapy.

ABSTRACT:

Objectives

To reveal the cellular composition and molecular environment of the inflammatory infiltrates in residual deep pockets of severe periodontitis following non-surgical periodontal therapy (NSPT). This may provide critical insights for targeted therapy in managing severe periodontitis.

Methods

Single-cell RNA-seq (scRNA-seq) and bulk RNA-seq of periodontal tissues from patients with severe periodontitis following NSPT and healthy donors were performed. Eligible public datasets of scRNA-seq and bulk RNA-seq were also used for analysis. Multiplex immunohistochemical immunofluorescence was used to validate the identified cell subtypes further.

Results

We identified a diverse inflammatory microenvironment landscape in the residual deep pockets of severe periodontit

SUBMITTER: Chen J 

PROVIDER: S-EPMC12523107 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature