Epigenetic regulation by WTAPP1 promotes trophoblast dysfunction in gestational diabetes via CNR1/NF-κB activation.

ABSTRACT: Gestational diabetes mellitus (GDM) poses significant risks to maternal and fetal health, necessitating exploration of novel molecular mechanisms. Clinical data from placental tissues from 33 GDM patients and 30 controls revealed Wilms tumor 1 associated protein (WTAP) pseudogene 1 (WTAPP1) was significantly upregulated in GDM, correlating with fasting glucose levels. In vitro, high glucose (25 mM) triggered oxidative stress (manifested by increased MDA, reduced GSH/GSSG ratio, and decreased CAT and SOD activity), inflammation (evidenced by elevated secretion of IL-1β, IL-6, TNF-α, and MCP-1), and apoptosis (along with upregulated cleaved caspase-3 or Bax, and downregulated Bcl-2) in trophoblast cell HTR-8/Svneo, while WTAPP1 knockdown partially restored viability, reduced apoptosis, and m

SUBMITTER: Yang N 

PROVIDER: S-EPMC12523184 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature