Green Extraction and Targeted LC-MS Analysis of Biopesticides in Honey Using Natural Deep Eutectic Solvents.

ABSTRACT: Natural Deep Eutectic Solvents (NADES) were synthesized from food-grade components and evaluated as green extractants for the simultaneous recovery and liquid chromatography coupled to quadrupole-Orbitrap mass spectrometry (LC-Q-Orbitrap-MS) analysis of biopesticide residues in a complex matrix like honey. Conventional solid-liquid extraction (SLE) was applied, initially using choline chloride-2,3-butanediol (1:4, molar ratio) as the NADES extractant solvent, before systematically evaluating other NADES formulations. Extraction parameters, such as time (10 min, 20 min, and 30 min), technique (rotary mixing vs. sonication), and NADES composition, namely lactic acid-glucose-water (LGH, 5:1:9, molar ratio), lactic acid-glycerol-water (LGLH, 1:1:3, molar ratio), urea-glycerol-water (UGLH, 1:1:

SUBMITTER: Ravi T 

PROVIDER: S-EPMC12523273 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature