Valorization of Tomato Leaves: Optimization of Eco-Friendly Phenolic Extraction and Assessment of Biological Activities.

ABSTRACT: Tomato leaves, typically discarded during harvest, are a rich yet underutilized source of bioactive compounds. This study aimed to valorize tomato leaves by optimizing the extraction of their phenolic compounds using a water-based method and response surface methodology. The optimal conditions, notably heating a mixture of 1:50 solid-to-liquid ratio at 71 °C for 29 min, yielded the most total phenolic content and antioxidant activity. The biological activities of the lyophilized tomato leaf extract (TLE) were then assessed. TLE showed dose-dependent antimicrobial activity against Escherichia coli and Candida albicans, but neither against Pseudomonas aeruginosa nor Staphylococcus aureus. In addition, it demonstrated moderate cytotoxicity against MCF-7 breast canc

SUBMITTER: Helmi L 

PROVIDER: S-EPMC12523334 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature