Gadd45B Deficiency Drives Radio-Resistance in BRAF^V600E-Mutated Differentiated Thyroid Cancer by Disrupting Iodine Metabolic Genes.

ABSTRACT:

Background

Differentiated thyroid cancer (DTC) is commonly treated with radioactive iodine (RAI), but resistance to RAI remains a significant clinical challenge. The molecular mechanisms driving dedifferentiation and RAI refractoriness, particularly in BRAF^V600E-mutated tumors, are not fully understood.

Methods

RNA sequencing was conducted on BRAF^V600E-mutated DTC and RAIR-DTC tissue samples to identify differentially expressed genes. Gadd45B was identified as significantly downregulated in RAIR-DTC. Functional studies including overexpression and knockdown experiments were performed in thyroid cancer cell lines and xenograft models. Downstream targets, including MAP3K4 and MYCBP, were evaluated through co-immunoprecipitation, luciferase assays, and Wes

SUBMITTER: Jiang S 

PROVIDER: S-EPMC12523365 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature