Cryopreservation increases sperm DNA fragmentation in normozoospermic Libyan men: the role of oxidative stress and the protective effect of melatonin.

ABSTRACT: Cryopreservation of sperm is routinely used in assisted reproduction technology (ART) for male fertility preservation. However, this method has been associated with oxidative stress and DNA fragmentation that may impair sperm quality. Additionally, antioxidant interventions such as melatonin supplementation have not been thoroughly explored in this setting. Although Libya is reported to have one of the highest global prevalence rates of male infertility, Libya-specific data remain limited. This study aimed to determine the effect of a single freeze-thaw cycle on sperm DNA fragmentation and oxidative stress markers, and to evaluate whether melatonin has an impact on post-thaw oxidation profiles. This prospective cohort study was conducted at the Fertility and Reproductive Medicine Center, B

SUBMITTER: Al Bijou AA 

PROVIDER: S-EPMC12523456 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature