Metabolomic Analysis of Environmental Biomarkers Reveals Markers of Mate Preference in Female Giant Pandas.

ABSTRACT: The giant panda (Ailuropoda melanoleuca) is a vulnerable animal in China, and it is crucial to improve the reproduction efficiency of the giant panda. Mate preference is an important part of natural mating. We hypothesized that AGS metabolites differ according to their mate preference. In this study, we determined estrus-associated hormone levels in the urine of 19 female giant pandas. After confirming estrus via hormone levels and behavioral observation, we collected environmental biomarkers for metabolomics analysis. A total of 19 samples were divided to two groups according to the mating preference of female giant pandas. Metabolomics analysis by LC-MS/MS showed that a total of 115 differentially expressed metabolites were identified, including 97 upregulated metabolites and 18 d

SUBMITTER: Feng Y 

PROVIDER: S-EPMC12523548 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature