Acid-Adapted Polyphenol Oxidases from Agricultural Wastes: Extraction, Characterization, and Application in Plant Protein Crosslinking.

ABSTRACT: Polyphenol oxidases (PPOs) are enzymes that oxidize mono- and diphenolic compounds to o-quinones, facilitating pigment formation and protein crosslinking in food systems, thereby improving their techno-functional properties. However, most PPOs function optimally near neutral pH, limiting their application in acidic food products. This study aimed to extract acid-adapted PPOs from various fruit by-products, including Hass avocado seeds (pH 5.9), Anjou pears (pH 4.0), Bartlett pears (pH 4.0), Red Delicious apples (pH 4.0), and McIntosh apples (pH 3.3), and characterize PPO properties and its substrate specificity using colorimetric assay. SDS-PAGE was used to assess PPOs' molecular weight and PPOs' capacity for plant protein crosslinking. The results showed that PPOs from Anjou and Bartlett

SUBMITTER: Tran T 

PROVIDER: S-EPMC12523565 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature