Analysis of the Role of the SRC Tyrosine Kinase and Podoplanin in the Process of Entosis.

ABSTRACT:

Background

Over the last years, the phenomenon of entosis, a form of cell-in-cell structure, has been highlighted in various tumors, including poorly treatable breast or pancreatic cancers. Nevertheless, not only the biological properties, but also the molecular drivers of entosis remain unclear. Here, we evaluated SRC tyrosine kinase, a key proto-oncogene, and podoplanin (PDPN), a membrane glycoprotein, as potential regulators of entotic cell formation.

Methods

In the study, two entosis-competent cell lines, BxPC-3 and MFC-7, originating from pancreatic and breast cancers, respectively, were used. SRC or PDPN genes were silenced using dedicated siRNA and the frequency of entotic structure formation was assessed using fluorescent staining and confocal imaging.<

SUBMITTER: Gawel AM 

PROVIDER: S-EPMC12523579 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature