Quercetin Increases Expression of Membrane-TRAIL in Glioblastoma Cells Resulting in Apoptosis.

ABSTRACT:

Background/objectives

Glioblastoma isocitrate dehydrogenase (IDH)-wild type (GBM) belongs to a deadly class of cancers with a limited number of effective therapies and a dismal prognosis. Quercetin is a natural flavonoid with proven anti-cancer effects. This study aimed to assess the effect of quercetin on recombinant human tumor necrosis factor-related apoptosis-inducing ligand (rhTRAIL)-mediated apoptosis in various GBM cells and control astrocytes.

Methods

Two astrocyte cell lines and three GBM cell lines, M059K, T98G, and A172, were treated with quercetin (±rhTRAIL), and the results were evaluated by Western blotting, confocal microscopy, and flow cytometry analyses.

Results

Quercetin alone did not induce apoptosis in normal astrocytes. Surprisingly, quercetin alo

SUBMITTER: Thorpe EM 

PROVIDER: S-EPMC12523679 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature