Construction of Composite Biocontrol Agent (BCA): Developing Effective Strategies for Controlling Postharvest Blue Mold and Patulin in Apples.

ABSTRACT: Postharvest blue mold in apples, caused by Penicillium expansum, leads to fruit decay and patulin (PAT) contamination, incurring major economic and health risks. This study developed a composite biocontrol agent (BCA) by co-cultivating three antagonistic yeasts (Meyerozyma caribbica, Metschnikowia zizyphicola, and Pichia rarassimilans). Mixed-culture conditions and protective additives formulation were optimized via response surface methodology. Optimal biomass production was achieved with a 1:2:3 (v/v/v) yeast ratio in medium containing sucrose (12.49 g/L), yeast extract powder (13.3 g/L), K₂HPO₄ (0.88 g/L), and NaCl (0.95 g/L) under pH 7.0, 1% total inoculum concentration, 24 °C, and a 60 h incubation. The liquid BCA

SUBMITTER: Cong L 

PROVIDER: S-EPMC12523723 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature