Efficient synthesis of l-DOPA in <i>Escherichia coli</i> via cofactor and enzyme engineering.

ABSTRACT: The global incidence of Parkinson's disease continues to rise. Levodopa (l-DOPA) is the core therapeutic drug, and efficient and sustainable production methods are needed. However, the complex metabolic pathways and the low catalytic efficiency of enzymes limit biosynthesis of l-DOPA in microorganisms. To address this issue, this study significantly enhanced the production efficiency of l-DOPA through a multi-dimensional, integrated metabolic and enzyme engineering approach. Firstly, the de novo synthesis pathway for l-DOPA was established through optimization of the promoter, ribosome-binding site (RBS), plasmid copy number, and tighly accurately regulating the expression level of key enzymes. Secondly, combined with metabonomic analysis, carbon metabolic flow was diverted, increas

SUBMITTER: Deng L 

PROVIDER: S-EPMC12523793 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature