In Silico Analysis of Tat Exons to Increase the Efficacy of a Nef-Tat-based HIV-1 Vaccine Candidate.

ABSTRACT:

Background

The global human immunodeficiency virus HIV/AIDS pandemic persists without complete eradication. Developing a safe and effective vaccine remains the most promising approach, but ongoing clinical trials have been unsuccessful due to the vaccines' inability to stimulate robust immunity.

Objectives

The present research endeavor proposes an innovative therapeutic vaccine by employing immunoinformatics strategies. Herein, we aimed to compare the efficiency of the whole sequence of Tat^{(exons 1 + 2)} with its first exon^{(exon 1)} in a fusion vaccine construct harboring the whole sequence of the Nef protein [i.e., Nef-Tat^{(exons 1 + 2)} and Nef-Tat^{(exon 1)} fusion proteins] using in silico studies.

Methods

First, the secondary

SUBMITTER: Sadeghi L 

PROVIDER: S-EPMC12523967 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature