Quantitative analysis of human umbilical vein endothelial cell morphology and tubulogenesis.

ABSTRACT: Primary human umbilical vein endothelial cells can grow as both a monolayer in culture and also as a capillary-like network making them an ideal model system in order to study vascular remodelling. Image-based analysis can allow assessment of cell morphology and motility but is dependent on accurate cell segmentation which requires high-contrast images not normally achievable without fluorescent markers. Here, ptychography is employed as a label-free image-based modality in order to extract quantitative metrics of morphology and tubulogenesis from cultured HUVECs over time in an automated multiwell assay. Phase-specific parameters of dry mass, optical thickness and sphericity were extracted and assessed alongside other metrics of cell number and shape. Tubulogenesis could be captured dynam

SUBMITTER: Mignone V 

PROVIDER: S-EPMC12523982 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature