Matrix Interference of Vegetable on Enzyme-Linked Immunosorbent Assay for Parathion Residue Detection.

ABSTRACT: Complex matrix of vegetable severely interferes with enzyme-linked immunosorbent assay (ELISA) accuracy, limiting its application in parathion residue detection. This study investigated the interference mechanism of vegetable matrix, including chlorophyll, perilla protein, glucose, fructose, and sucrose, on ELISA. Furthermore, we validated the vegetable matrix interference on parathion residue ELISA by comparing the matrix interference index (I_m) and recovery rate of vegetable samples before and after acetic acid-treatment. The results demonstrate that the addition of vegetable matrix significantly interferes with ELISA, with the antibody-IgG-HRP binding being subject to the most pronounced interference. Compared to the I_m (16-26%) of non-acetic acid treatment, the I<

SUBMITTER: Chen L 

PROVIDER: S-EPMC12524109 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature