Goblet Cells and Mucus Composition in Jejunum and Ileum Containing Peyer's Patches and in Colon: A Study in Pigs.

ABSTRACT: The intestinal mucus layer is a dynamic protective barrier that maintains gut homeostasis, supports immune defense, and regulates host-microbiota interactions. Rodent models have yielded valuable insights, but their intestinal structure and physiology differ from those of humans and pigs. By contrast, the omnivorous pig shares closer anatomical and immunological features with humans, making it a relevant large-animal model in translational studies. In this study, we established a histological workflow for porcine intestine by combining Carnoy's fixation with Alcian Blue-Periodic Acid-Schiff and Mucicarmine staining. This enabled accurate visualization and quantification of goblet-cell density and mucus thickness across intestinal segments, with a particular focus on Peyer's patches-key sit

SUBMITTER: Ginoski V 

PROVIDER: S-EPMC12524252 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature