Intestinal <i>Myo</i>-Inositol Metabolism and Metabolic Effects of <i>Myo</i>-Inositol Utilizing <i>Anaerostipes rhamnosivorans</i> in Mice.

ABSTRACT: The gut microbiome is strongly implicated in the development of obesity and type 2 diabetes mellitus (T2DM). A recent study demonstrated that 6-week oral supplementation of Anaerostipes rhamnosivorans (ARHAM) combined with the prebiotic myo-inositol (MI) reduced fasting glucose levels in mice. In the present study, we investigated the effects of a 13-week ARHAM-MI supplementation in high-fat diet-fed mice and examined the metabolic fate of MI, including its microbial conversion into short-chain fatty acids (SCFAs), using ¹³C-MI and stable isotope tracers in the cecum, portal vein, and peripheral blood. The results showed that the ARHAM-MI group gained less weight than the MI-only and placebo groups. Analysis of intestinal mRNA and stable isotope tracing revealed th

SUBMITTER: Grefhorst A 

PROVIDER: S-EPMC12524588 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature