Effect of Nickel Stress on Nitrogen Metabolism in Cucumber Plants.

ABSTRACT: Excessive concentrations of nickel (Ni) are phytotoxic, leading to disturbances in plant cell structure and function. Although some attempts have been made to elucidate the Ni impact on plant metabolism, the effect of this metal on nitrogen assimilation and transformation of nitrogen compounds still remains poorly understood. The objective of our study was to gain a better insight into the Ni influence on nitrogen metabolism in cucumber plants. Nitrogen metabolism-related enzyme activities and selected metabolite contents were assayed using spectrophotometric methods. Additionally, in the leaves, nitrogen assimilation-involved gene expression was analyzed using quantitative real-time PCR. Nickel treatment resulted in a decline in NO3- content in the leaf and NH4+ content in the root. In th

SUBMITTER: Gajewska E 

PROVIDER: S-EPMC12524678 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature