CRISPR/Cas9-Mediated Targeted Mutagenesis of <i>GmAS1/2</i> Genes Alters Leaf Shape in Soybean.

ABSTRACT: ASYMMETRIC LEAVES1 (AS1) and AS2 play essential roles in regulating leaf development in plants. However, their functional roles in soybean remain poorly understood. Here, we identified two members of the soybean AS1 gene family, GmAS1a and GmAS1c, which exhibit high expression levels in stem and leaf tissues. Using the CRISPR/Cas9 system, we targeted four GmAS1 and three GmAS2 genes, generating mutant lines with distinct leaf development phenotypes, including wrinkling (refers to fine lines and creases on the leaf surface, like aged skin texture), curling (describes the inward or outward rolling of leaf edges, deviating from the typical flat shape), and narrow. We found that functional redundancy exists among the four GmAS1 gen

SUBMITTER: Xu J 

PROVIDER: S-EPMC12524711 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature