Development of Melting-Curve-Based Real-Time PCR for Differentiating Medically Important <i>Candida</i> Species.

ABSTRACT: Candida species are the primary fungal pathogens of invasive infections associated with high morbidity and mortality. The identification of these microorganisms is critical for therapeutic management and control of hospital infection. Herein, assays targeting the Intergenic Spacer 2 (IGS2) and Internal Transcribed Spacer 1 (ITS1) from the rDNA locus were developed to differentiate Candida species. Based on consensus nucleotide sequences, specific primers and positive controls were designed, and standard PCR and real-time PCR (qPCR) assays were performed. All primers resulted in specific amplification of the molecular targets of each species with no amplifications of the negative template control. Furthermore, the primers were highly specific when tested with a range of fungal

SUBMITTER: Tavares ER 

PROVIDER: S-EPMC12524715 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature