Challenges in Applying DNA-Binding Protein Predictors to Biological Research.

ABSTRACT: DNA binding proteins play a crucial role in regulating gene expression, DNA replication, and chromatin organization. While many DNA-binding proteins have been identified, many unique DNA-binding proteins in non-model organisms and recently evolved lineage- or species-specific proteins remain uncharacterized or often lack experimental validation. In addition, genetic variants may alter previously known DNA-binding proteins, leading to loss of binding ability. To address this gap, various computational tools have been developed to predict DNA-binding proteins from protein sequences or structures. Yet, their real-world utility in biological research remains uncertain. To evaluate their effectiveness, we assessed the availability and predictive performance of existing tools using five real-wor

SUBMITTER: Cowgill G 

PROVIDER: S-EPMC12524727 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature