Ultra-Fast Intraoperative <i>IDH</i>-Mutation Analysis Enables Rapid Stratification and Therapy Planning in Diffuse Gliomas.

ABSTRACT: Diffuse gliomas are the most common primary brain tumors in adults in the Western world. According to the 2021 World Health Organization (WHO) classification of central nervous system (CNS) tumors, the assessment of isocitrate dehydrogenase (IDH1/2)-mutation status is essential for accurate patient stratification. In this study, we performed a comprehensive evaluation of IDH-mutation status in the intraoperative setting using the Idylla platform. The reference cohort comprised 30 formalin-fixed paraffin-embedded (FFPE) tissue samples with known IDH status, while the exploration cohort included 35 intraoperative snap-frozen and native-tissue specimens. The results were compared with those of a standard next-generation sequencing (NGS) analysis. Our findings demonstrate

SUBMITTER: Kraus TFJ 

PROVIDER: S-EPMC12524806 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature