<i>Octopus minor</i> Antimicrobial Peptide-Loaded Chitosan Nanoparticles Accelerate Dermal Wound Healing.

ABSTRACT: Octominin is a peptide derived from the Octopus minor defense protein, which has shown antimicrobial and immunomodulatory properties. The present study describes the efficacy of Octominin-encapsulated chitosan (CN) nanoparticles (Octominin-CNPs) on in vitro and dermal wound healing in zebrafish. Initial viability analysis revealed there was no significant toxicity of Octominin-CNPs up to 200 μg/mL in human dermal fibroblast (HDF) cells and in zebrafish larvae (up to 50 μg/mL). Moreover, the potential wound healing activity of Octominin-CNPs was observed using the cell-scratch assay. In the in vivo study, wounded adult zebrafish were applied with the appropriate treatment (PBS, CNPs, Octominin, and Octominin-CNPs) 20 μg/wound/fish as a topical application at 0, 2, and 4 days post-wou

SUBMITTER: Dias MKHM 

PROVIDER: S-EPMC12525058 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature