Characterization of Rhesus Macaque Embryonic Stem Cells in Primed and Naive-like Cell States of Pluripotency Using Fourier Transform Infrared (FTIR) Microspectroscopy.

ABSTRACT: We evaluated the potential of Fourier-transform infrared (FTIR) microspectroscopy for non-invasive biochemical profiling of rhesus macaque embryonic stem cells (rhESCs) cultured in either conventional FGF2/KOSR medium or a novel formulation, ALGöX. Cells from both conditions were analyzed by immunocytochemistry, RNA sequencing, and high-resolution FTIR profiling. Molecular marker expression patterns and transcriptional profiles revealed that rhESCs maintained in FGF2/KOSR were in the primed pluripotent state, whereas those cultured in ALGöX adopted a naïve-like state. FTIR spectra showed consistent differences in protein, lipid, and nucleic acid signatures, with ALGöX-cultured cells displaying higher amide I/II and nucleic acid absorbance and FGF2/KOSR-cultured cells exhibiting stronger li

SUBMITTER: Srisutush J 

PROVIDER: S-EPMC12525272 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature