<i>OsPIP2;1</i> Positively Regulates Rice Tolerance to Water Stress Under Coupling of Partial Root-Zone Drying and Nitrogen Forms.

ABSTRACT: The coupling of partial root-zone drying (PRD) with nitrogen forms exerts an interactive "water-promoted fertilization" effect, which enhances rice (Oryza sativa L.) growth and development, improves water use efficiency (WUE), mediates the expression of aquaporins (AQPs), and alters root water conductivity. In this study, gene cloning and CRISPR-Cas9 technologies were employed to construct overexpression and knockout vectors of the OsPIP2;1 gene, which were then transformed into rice (cv. Meixiangzhan 2). Three water treatments were set: normal irrigation (CK); partial root-zone drying (PRD); and 10% PEG-simulated water stress (PEG), combined with a nitrogen form ratio of ammonium nitrogen (NH₄⁺) to nitrate nitrogen (NO₃^-) at 50:50

SUBMITTER: Kuang C 

PROVIDER: S-EPMC12525400 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature