Genome-Wide Isoform Switching Reveals SR45-Mediated Splicing Control of <i>Arabidopsis</i> Leaf Senescence.

ABSTRACT: Leaf senescence is the final, programmed stage of leaf development, marked by nutrient remobilization and tightly regulated molecular events. Although alternative splicing has emerged as a major regulator of plant development, its role in isoform switching during leaf aging remains poorly understood. To address this, we conducted a genome-wide analysis of isoform switching in Arabidopsis, leveraging publicly available RNA-seq data from mature (16-day-old) and senescent (30-day-old) leaves, analyzed with the IsoformSwitchAnalyzeR package. Between these two developmental stages, we identified 269 genes exhibiting 377 significant isoform switches collectively predicted to alter protein localization, coding potential, and transcript stability. Experimental validation confirmed pr

SUBMITTER: Albaqami M 

PROVIDER: S-EPMC12525425 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature