Immunoglobulin G Subclass-Specific Glycosylation Changes in Rheumatoid Arthritis.

ABSTRACT: Rheumatoid arthritis (RA) is the most common inflammatory polyarthritis. In addition, 60-80% of patients express anti-citrullinated protein antibodies (ACPAs), which serve as a diagnostic marker for RA. The effector functions of these autoantibodies can be heavily affected by the N-glycosylation of their Fc region. Here we present a comparison of the Fc N-glycosylation of ACPA IgG to that of non-ACPA IgG from the same patients, and of healthy controls, in an IgG isoform-specific manner. We isolated ACPA and normal serum IgG, digested by trypsin, and separated the resulting peptide mixture by a reversed-phase nanoLC coupled to a Bruker Maxis II Q-TOF, and determined the relative abundance of glycoforms. The paired analysis of galactosylation and sialylation of the IgG subclasses of ACPA and

SUBMITTER: Szabo D 

PROVIDER: S-EPMC12525468 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature