Parallel Three-Dimensional Tracking of Quantum Rods Using Polarization-Sensitive Spectroscopic Photon Localization Microscopy.

ABSTRACT: Semiconductor nanocrystals and their variants are widely used in biological research as fluorescent probes. Their unique characteristics, such as intense brightness, tunable emission properties, and resistance to photobleaching, make them ideal candidates for single-molecule imaging and tracking with localization precision far beyond the diffraction limit. Their fluorescence polarization states and emission spectra can be further utilized to probe changes in their mechanical properties and residing nanoenvironments. We developed a three-dimensional (3D), polarization-sensitive, spectroscopic photon localization microscopy (3D-Polar-SPLM) that enables parallel 3D tracking of individual quantum rods (QRs) while simultaneously capturing their fluorescence spectra and polarization states. Usin

SUBMITTER: Dong B 

PROVIDER: S-EPMC12525470 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature