Development and Validation of HPLC-DAD/FLD Methods for the Determination of Vitamins B₁, B₂, and B₆ in Pharmaceutical Gummies and Gastrointestinal Fluids-In Vitro Digestion Studies in Different Nutritional Habits.

ABSTRACT: Two HPLC-based analytical methods, one with DAD and the other with an FLD detector, were developed and validated for the simultaneous analysis of B₁, B₂, and B₆ vitamins, both in pharmaceutical gummies and in gastric and intestinal fluids (with water or milk or orange juice). For the detection of B₁ by fluorometry, a pre-column oxidation/derivatization process was accomplished in the presence of B₂ and B₆ vitamins. The methods were performed on an Aqua column (250 mm × 4.6 mm, 5 mm) at 40 °C, with isocratic elution (70% NaH₂PO₄ buffer pH 4.95 and 30% methanol) and a flow rate of 0.9 mL/min. Both were validated according to ICH specifications in terms of linearity (R² > 0.999), accuracy (% Mea

SUBMITTER: Kamaris G 

PROVIDER: S-EPMC12525618 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature