Targeted Enrichment and Characterization of Diester Diterpenoid Alkaloids in <i>Aconitum</i> Herbs Using Gas-Liquid Microextraction Coupled with High-Resolution Mass Spectrometry.

ABSTRACT: Diterpenoid diester alkaloids (DDAs) are the primary toxic constituents in aconite herbs, while also being the key pharmacologically active components. Consequently, establishing rapid enrichment and highly sensitive analytical methods for DDAs is of critical importance. Herein, we developed and constructed a gas-liquid microextraction (GLME) device, which enables the rapid and selective enrichment of DDAs from complex matrices. The enriched extract can be directly analyzed by high-resolution Orbitrap mass spectrometry without requiring any further pretreatment. A comparative analysis of six commonly used Aconitum herbs medicines and their processed derivatives was conducted. Notably, GLME enhanced the mass spectrometric signals of DDAs by 3-4 orders of magnitude, facilitating the i

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12525639 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature