Development of a Validated High-Performance Thin-Layer Chromatography (HPTLC) Analysis Protocol for Salivary Caffeine Used as a Probe Drug.

ABSTRACT: CYP1A2 activity plays a critical role in the metabolism of drugs such as caffeine, clozapine, propranolol, and warfarin. In pharmacogenomic studies, caffeine is a probe drug of choice for CYP1A2 phenotyping. Due to the non-invasive nature of sampling, saliva is an alternative biofluid to plasma for monitoring caffeine levels. This study reports on a validated HPTLC method for quantifying salivary caffeine levels, which can support future studies on CYP1A2 phenotyping employing caffeine as a probe drug. The HPTLC method, using silica gel 60 F254 plates and acetone/toluene/chloroform (4:3:3, v/v/v) as the mobile phase, has detection and quantification limits of 2.42 and 7.34 ng/band, respectively. An optimised saliva processing protocol using a 1:1 dilution with methanol

SUBMITTER: Sikdar KMYK 

PROVIDER: S-EPMC12525945 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature