Identification and Functional Analysis of Two UGT84 Glycosyltransferases in Flavonoid Biosynthesis of <i>Carthamus tinctorius</i>.

ABSTRACT: Safflower (Carthamus tinctorius L.) is a multipurpose economic crop. Flavonoid glycosides are its key bioactive constituents, and several glycosyltransferases involved in their biosynthesis have been identified. The glycosyltransferase 84 subfamily represents a specialized branch with diverse functions, involved not only in catalyzing flavonoid glycosylation but also in the biosynthesis of auxins, tannins, and other compounds. However, this subfamily remains poorly characterized in safflower. In this study, two UGT84 subfamily genes, UGT84A28 and UGT84B3, were screened based on expression patterns and phylogenetic evolution analysis. Recombinant proteins were induced and purified using prokaryotic expression systems. Functional characterization was subsequently conduct

SUBMITTER: Ren C 

PROVIDER: S-EPMC12525976 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature