A semi-selective differential medium for identifying strains of Pantoea from environmental samples.

ABSTRACT: The genus Pantoea of the Erwinaceae is a genetically and metabolically diverse group whose representatives span a broad range of clinical and nonclinical environments. We sought to develop a culturing medium to facilitate the identification of Pantoea from environmental samples, and to distinguish its members from other closely related Gram-negative genera. We developed a semiselective differential medium, Pantoea Differential Medium (PDM), which contains crystal violet, sodium citrate, tryptone, as well as protease peptone. The efficacy of the medium was evaluated by assessing the growth and phenotype of 42 bacterial strains, including 18 strains of Pantoea along with other representatives of the Enterobacterales. All Pantoea strains, as well as Kosakonia sp. and Duffyella gerundensis for

SUBMITTER: Bateman A 

PROVIDER: S-EPMC12526038 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature