Structural basis of enhanced stalling efficiency of an engineered ribosome arrest peptide.

ABSTRACT: Ribosome stalling is a critical regulatory mechanism in protein synthesis, controlling the rate and fidelity of translation. Arrest peptides, short sequences within nascent chains, can induce ribosome stalling, providing insights into the dynamics of translation and potential therapeutic targets. In this study, we investigated the molecular mechanisms of ribosome stalling induced by an engineered ribosomal arrest peptide (eRAP). We used cryo-electron microscopy and biochemical assays to characterize the interactions between eRAP, the ribosome, and accessory factors such as the trigger factor. Our results reveal intricate details of the eRAP-induced ribosome stalling, including the conformational changes in the ribosomal tunnel and the nascent chain. We also observed interactions between eR

SUBMITTER: Sriramoju MK 

PROVIDER: S-EPMC12526060 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature