Charting the single-cell and spatial landscape of IDH-wild-type glioblastoma with GBmap.

ABSTRACT:

Background

Glioblastoma (GB), particularly IDH-wild type, is the most aggressive brain malignancy with a dismal prognosis. Despite advances in molecular profiling, the complexity of its tumor microenvironment and spatial organization remains poorly understood. This study aimed to create a comprehensive single-cell and spatial atlas of GB to unravel its cellular heterogeneity, spatial architecture, and clinical relevance.

Methods

We integrated single-cell RNA sequencing data from 26 datasets, encompassing over 1.1 million cells from 240 patients, to construct GBmap, a harmonized single-cell atlas. High-resolution spatial transcriptomics was employed to map the spatial organization of GB tissues. We developed the Tumor Structure Score (TSS) to quantify tumor organization and c

SUBMITTER: Ruiz-Moreno C 

PROVIDER: S-EPMC12526130 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature