Testcross Analysis of <i>Pl-1</i> Marker Expression and Seedling Vigor in Thai Maize Germplasm for Doubled Haploid Breeding Applications.

ABSTRACT: Doubled haploid (DH) technology accelerates maize breeding by generating completely homozygous lines within two generations, but its efficiency depends on reliable haploid identification markers. The Purple plant 1 (Pl-1) root pigmentation marker has emerged as a promising alternative to R1-Navajo (R1-nj), which suffers from frequent suppression in tropical germplasm. This study evaluated Pl-1 expression and seedling vigor in 298 diverse Thai maize genotypes across four market classes under controlled growth chamber conditions (24 ± 1 °C, 85-90% humidity, and standardized lighting), followed by testcross analysis with 89 representative genotypes crossed with BHI306 to distinguish between allelic absence and epistatic suppression mechanisms. Complete absence of Pl-1<

SUBMITTER: Thawarorit A 

PROVIDER: S-EPMC12526231 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature