Gastrointestinal Survivability of a BSH-Positive <i>Lacticaseibacillus rhamnosus</i> VB4 Strain and Its Effect on Bile Acid Deconjugation in a Dynamic In Vitro Gut Model.

ABSTRACT:

Background

Bile salt hydrolase (BSH) is a key probiotic trait, as it facilitates both host metabolism and bacterial survival into the gastrointestinal tract (GIT), through bile acid (BA) deconjugation, keeping intestinal homeostasis.

Objectives

The present study aims to investigate the viability of the Lacticaseibacillus rhamnosus VB4 strain and its effects on bile acid deconjugation during the gastrointestinal tract (GIT) passage, under a fed condition, using the in vitro SHIME^® (Simulator of the Human Intestinal Microbial Ecosystem) model.

Methods

Gastric, small intestinal and colonic fractions were monitored and a fecal slurry from a healthy donor was inoculated into the colonic compartment to establish the intestinal microbiota. Samples were coll

SUBMITTER: Vaccalluzzo A 

PROVIDER: S-EPMC12526281 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature