Bacterial Genomics for National Antimicrobial Resistance Surveillance in Cambodia.

ABSTRACT:

Background

Antimicrobial resistance (AMR) surveillance in low- and middle-income countries (LMICs) often relies on poorly resourced laboratory processes. Centralized sequencing was combined with cloud-based, open-source bioinformatics solutions for national AMR surveillance in Cambodia.

Methods

Blood cultures growing gram-negative bacteria were collected at 6 Cambodian hospitals (January 2021 to October 2022). Isolates were obtained from pure plate growth and shotgun DNA sequencing performed in country. Using public nucleotide and protein databases, reads were aligned for pathogen identification and AMR gene characterization. Multilocus sequence typing was performed on whole-genome assemblies and haplotype clusters compared against published genomes.

Results

Genes ass

SUBMITTER: Yek C 

PROVIDER: S-EPMC12526891 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature