Integrating 3D imaging, GWAS, and single-cell transcriptome approaches to elucidate root system architecture in Populus.

ABSTRACT: Roots are essential for nutrient uptake and structural stability in trees. Despite their critical role, the genetic determinants underlying root system architecture (RSA) remain poorly understood. In this study, we employed an integrated approach combining automated 3-dimensional (3D) spatial imaging, multiomics analyses, genetic transformation, and molecular experiments to investigate the genetic architecture and regulatory networks governing RSA in Simon poplar (Populus simonii). Here, using a panel of 303 P. simonii accessions collected from different geographical regions in China, we performed a genome-wide association study (GWAS) on 96 RSA traits and identified S-phase kinase-associated protein 2B (PsiSKP2B) as a candidate gene colocalized by 6 traits. By integrating the findings fro

SUBMITTER: Li J 

PROVIDER: S-EPMC12526957 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature