Visualization of Trypanosoma brucei flagellar pocket collar biogenesis identifies two new cytoskeletal structures.

ABSTRACT: Understanding how cells assemble internal structures is central to cell biology. In Trypanosoma brucei, the flagellar pocket (FP) is essential for nutrient uptake, and immune evasion, and its formation depends on a cytoskeletal structure called the flagellar pocket collar (FPC). However, the mechanisms underlying FPC assembly remain poorly understood. In this study, we used cutting-edge ultrastructure expansion microscopy (U-ExM) to investigate FPC biogenesis in T. brucei. We mapped the formation of the proximal part of the new microtubule quartet (nMtQ) alongside flagellum growth, providing new insights into its assembly. Additionally, we tracked the localization dynamics of key structural proteins-BILBO1, MORN1, and BILBO2-during the biogenesis of the FPC and the hook complex (HC). Notab

SUBMITTER: Zelena M 

PROVIDER: S-EPMC12527162 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature